This study was conducted to evaluate effects of various eco light sources with various lighting distance in ‘Viking’ rose (Rosa spp.) on the growth and flowering quality to be applied for farm sites. Treatment included 10-, 20-, and 30-RL (-BL, -RBL, -FL, and -IL), which referred to red LED (blue LED, red+blue LED, fluorescent, and incandescent) lighting at 10 cm, 20 cm, 30 cm respectively, apart from flowers. NL referred to natural light as a control. Growth and flowering of ‘Viking’ rose were non-destructively measured at 4, 6, and 8 weeks after treatment (WAT). FL treatment increased plant height at 4, 6, and 8 WAT, regardless of lighting distance, with the shortest height observed for the NL-treated flowers. 30 RL treatment also increased plant height at 6 and 8 WAT. Stem diameter and number of leaves were not significantly different for all the treatments at 8 WAT, with the lowest values observed for RBL treated-flowers among the light source treatments. Number of root was the greatest for the 30 BL-treated flowers (10.0) but the fewest for the 30 FL (4.7). Length of flower neck at 6 WAT was the extended by 6~7 cm in the 10 FL and 20 FL treatments as well as by 5~6 cm in the 20 RL and 30 RL treatments, inducing 100% of flowering. NL increased a* (29) of flower color, with the lowest value (10) observed for 20 RL. All things considered, 30 RL would be the best interaction treatment of source and distance of eco light to improve plant height and flowering quality of ‘Viking’ rose.