논문 상세보기
pp.228-236
Background: Sedum takesimense Nakai has been used as folk medicine in Korea. The present study aimed to determine the biological activity of S. takesimense by investigating the anti-inflammatory effects of S. takesimense water extract (SKLC) on the lipopolysaccharide-induced inflammatory response in RAW 264.7 cells.
Methods and Results: Cytotoxicity of SKLC on RAW 264.7 cells was determinded by performing MTS assay was found to have no cytotoxic effect on RAW 264.7 cells at a concentration range of 62 - 500 ㎍/㎖. Further, pretreatment of SKLC inhibited lipopolysaccharide-induced nitric oxide (NO) production in a dose-dependent manner. To determined the inhibitory mechanisms of SKLC on inflammatory mediators, we assessed the inducible nitric oxide synthase (iNOS) and cyclooxygnease-2 (COX-2) pathways. The activities of these pathways were decreased in a dose-dependent manner by SKLC. The production of tumor necrosis factor- α (TNF-α), interleukin (IL)-1β‚ and IL-6 were also reduced.
Conclusions: These results suggest that the down regulation of iNOS, COX-2, TNF-α, IL-1β‚ and IL-6 expression by SKLC are mediated by the down regulation of nuclear factor-κB (NF-κB) activity, a transcription factor necessary for pro-inflammatory mediators. This might be the mechanism underlying the anti-inflammatory effects of SKLC.
재료 및 방법
1. 실험 재료
2. 시약
3. 세포주 및 세포배양
4. 세포 독성 평가 (MTS 분석)
5. Nitric oxide (NO) 농도 측정
6. Prostaglandin E2 (PGE2) 분석
7. Real-time PCR (mRNA 발현분석)
8. Immunoblot 분석
9. 면역형광염색 (immunofluorescence) 분석
10. Cytokines (TNF-α, IL-1β, IL-6) 측정
11. 통계처리
결 과
1. 섬기린초 물추출물 (SKLC)의 RAW 264.7 세포주에서 세포생존률에 미치는 영향
2. 섬기린초 물추출물 (SKLC)의 nitric oxide 생성 억제 효과
3. 섬기린초 물추출물 (SKLC)의 prostaglandin E2 (PGE2)생성 억제 효과
4. 섬기린초 물추출물 (SKLC)의 염증관련 사이토카인의 생성억제 효과
5. RAW 264.7 세포주에서 염증관련 단백질의 발현 감소효과
6. 섬기린초 물추출물 (SKLC)의 면역형광염색을 통한 IκB-αdegradation의 완화효과
고 찰
REFERENCES
