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구절초(Chrysanthemum zawadskii var. latilobum)에서 분리된 다당류의 대식세포 면역조절 활성 KCI 등재
Immuno-Modulatory Activities of Polysaccharides separated from Chrysanthemum zawadskii var. latilobum in Macrophage Cells
pp.431-437
Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to 128 μg/mL), and there was no cytotoxicity at a dose below 32 μg/mL. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-6, IL-1β) production in the CZPS treated group (32 μg/mL) were 6.5±0.12 μM (NO), 1252.8±79.85 (TNF-α), 305.4±29.41 (IL-6), and 683.3±59.71 (IL-1β), respectively, and they were significantly increased when compared to the control group; 2.2±0.03 μM (NO), 452.3±38.34 (TNF-α), 31.7±5.75 (IL-6), and 184.1±11.52 (IL-1β). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-κB expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-κB signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.
재료 및 방법
1. 재료
2. 구절초 조다당(CZPS) 추출
3. 세포배양
4. 세포 생존율 평가
5. Nitric oxide(NO) 유도능 평가
6. Cytokine 분비 유도능 평가
7. Western blot analysis
8. 통계분석
결과 및 고찰
1. CZPS의 대식세포 생존율에 미치는 영향
2. CZPS의 iNOS 관련 NO 생성에 미치는 영향
3. CZPS의 cytokine 분비능에 미치는 영향
4. CZPS의MAPKs 인산화및NF-κB의발현에미치는영향
References
