Phagocytosis is a primary and an essential step of host defense, and is triggered by the interaction of particles with specific receptor of macrophages. In this study, we investigated the effect of extracts of Rudbeckia laciniata (RLE) on the phagocytic activity of macrophage, by monitoring the phagocytosis-associated signal transduction. RLE markedly increased phagocytosis of serum-opsonized zymosan particles (SOZ), while phagocytosis of IgG-opsonized zymosan particles (IOZ) or none-opsonized zymosan particles (NOZ) remained unaffected. However, RLE did not affect the binding of opsonized zymosan particles (OZ) with the cell surface of macrophage. This suggests that RLE may regulate SOZ-induced intracellular signaling during phagocytosis of macrophage. To confirm this hypothesis, we investigated whether RLE was involved in the RhoA-mediated signal transduction during phagocytosis of SOZ. Inhibitors of the RhoA-mediated signaling pathway, such as Y-27632 (for ROCK), ML-7 (for MLCK), and Tat-C3 (for RhoA), totally blocked phagocytosis of SOZ enhanced by RLE, as well as phagocytosis of SOZ. Additionally, RhoA activity was markedly increased when cells were treated with RLE, suggesting that RLE could increase the phagocytic activity of macrophage via RhoA-ROCK/MLCK signal pathway. Thus, RLE may be used to develop functional foods for immunity.

서 론
 재료 및 방법
  1. 실험재료
  2. 삼잎국화 열수추출
  3. 세포 배양
  4. 세포 독성 실험
  5. 식균 자이모잔(Opsonized zymosan particles)의 준비
  6. 대식세포의탐식능과자이모잔의세포표면결합능조사
  7. 세포 분획(Subcellular fractionation)과 GST-pull downassay
  8. 통계처리
 결과 및 고찰
  1. 대식세포의 자이모젠 탐식능과 결합능에 있어서 삼잎국화 추출물의 효과
  2. 삼잎국화 추출물의 ROCK와 MLCK 신호기전 활성화
  3. 삼잎국화 추출물의 RhoA 신호기전 활성화
  4. 삼잎국화추출물처리에의한RhoA 단백질의활성변화
 요약 및 결론
 References

• 김준섭(한국교통대학교 생명공학과) | Jun-Sub Kim Corresponding author