The objective of the present investigation was to obtain vitamin, mineral, flavonoid, and polyphenol profiles of Rudbeckia laciniata (RL), and to examine the effects of extract of RL (RLE) on various physiological activities of HaCaT keratinocyte for the utilization of RL as natural raw materials to develop functional food. To accomplish this purpose, we checked the contents of the general nutrients of RL. The contents of vitamin A, vitamin B1 and vitamin B2 were 7.49 μg/g, 51.96 μg/g, and 132 μg/g respectively, while vitamin C and vitamin D3 were not detected. The contents of mineral such as Ca, K and Fe were 2.01 mg/g, 6.06 mg/g and 0.03 mg/g respectively. Total flavonoid contents of RLE were 0.25 mg/g, and total polyphenol were estimated as 1.43 mg/g. Because RL contains high levels of vitamin A which is associated with skin aging, we investigated the effect of RLE on physiological function of keratinocytes with respect to skin aging. We found that RLE significantly increased the growth rate of HaCaT cells and reduced ultraviolet radiation B (UVB)-induced cellular toxicity. Also, the extract of Rudbeckia laciniata attenuated the UVB-induced reactive oxygen species (ROS) generation in a dose-dependent manner in HaCaT cells. In addition, treatment with the extract dose-dependently increased migration activity of HaCaT cells. Thus, these findings indicated that RLE could regulate the physiological activity of keratinocytes, and may be used to develop functional foods.

서 론
 재료 및 방법
  1. 실험재료
  2. 삼잎국화 열수추출
  3. 삼잎국화 성분 분석
  4. 세포 배양
  5. 세포 독성 실험
  6. UVB 조사
  7. DPPH radical 소거 활성
  8. ROS 생성 실험
  9. HaCaT 세포의 이동능 조사
  10. 통계처리
 결과 및 고찰
  1. 삼잎국화의 성분 함량
  2. UVB 조사에 따른 피부세포 독성에서 삼잎국화 추출물의 효과
  3. 삼잎국화 추출물의 DPPH radical 소거 활성
  4. UVB에 의한 피부세포의 ROS 생성에 있어서 삼잎국화 추출물의 효과
  5. 피부세포의 이동능에 있어서 삼잎국화 추출물의 효과
 References

• 김준섭(한국교통대학교 생명공학과) | Jun-Sub Kim Corresponding author