This study describes results on sexual maturation and characteristics of natural spawned eggs to develop a method for the production of stable, healthy fertilized eggs from captive-reared yellowtail kingfish, Seriola lalandi. A total of 59 yellowtail kingfish were captured off the coast of Jeju Island, after which the broodstock was cultured in indoor culture tank (100 m3) until they were 6.1–14.9 kg in body weight. As part of the rearing management for induced sex maturation, the intensity of illumination was maintained at 130 lux. The photoperiod (light/dark; L/D) was set to a 12 L/12 D from October 2013 to January 2014, and 15 L/9 D from February 2014 to June 2014. Feeds comprised mainly EP (Extruded Pellets), with squid cuttlefish added for improvement of egg quality, and was given from April to June 2014. The first spawning of yellowtail kingfish occurred in May 3, 2014, at a water temperature of 17.0°C. Spawning continued until June 12, 2014, with the water temperature set at 20.5°C. Time of spawning was 26 times at this period. The total number of eggs that spawned during the spawning period was 4,449×103. The buoyant rate of spawning eggs and fertilization rate of buoyant eggs during the spawned period were 76.1% and 100%, respectively. The diameters of the egg and oil globule were 1.388 ± 0.041 mm and 0.378 ± 0.029 mm, respectively, which was higher in early eggs than in those from late during the spawned period.

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
  1. Growth of broodstock
  2. Spawning behavior
  3. Spawning amount
  4. Fertilized eggs
 DISCUSSION
 REFERENCES

• Sang Geun Yang(Jeju Fisheries Research Institute, National Institute of Fisheries Science)
• Seung Cheol Ji(Jeju Fisheries Research Institute, National Institute of Fisheries Science, Jeju 63610, Korea)
• Sang Gu Lim(Jeju Fisheries Research Institute, National Institute of Fisheries Science)
• Sang Woo Hur(2Marine Science Institute, Jeju National University)
• Minhwan Jeong(Jeju Fisheries Research Institute, National Institute of Fisheries Science)
• Chi Hoon Lee(Marine Science Institute, Jeju National University)
• Bong Seok Kim(Jeju Fisheries Research Institute, National Institute of Fisheries Science)
• Young-Don Lee(Marine Science Institute, Jeju National University) Corresponding Author