논문 상세보기
pp.47-52
The objective of this study was to investigate the result of in vivo embryo collection and pregnancy rate after embryo transfer using sex-sorted sperm of Korean brindle cattle. Donor Korean brindle cattle superovulation treated by decreasing dose of FSH injection. Embryos were recovered on 7 days after the third artificial insemination. Control group semen straw used artificial insemination contained 20 million sperm. Sex-sorted semen straws contained 4 million sperm or 10 million sperm. As for the result of the recovery of the in vivo embryos derived from sex-sorted sperm, the number of transferable embryos was significantly highly recovered to be 6.20±2.28/donor from the control group and was significantly lowly recovered to be 1.57±1.72/donor from the group treated at a sperm concentration of 10×106 (p<0.05). The number of unfertilized embryo was 0.8±1.30/donor in control group which was significantly lower than the group treated at a sperm concentration of 4×106 (p<0.05). However, there was no significant difference in the number of undeveloped ova between control and treatment groups. Pregnancy rate after embryo transfer was shown to be 35.00% in control group and 12.50% in treatment group. The karyotype analysis of the calf derived from sex-sorted sperm resulted in a similar chromosomal distribution pattern (2n=60, XX) compared to those of common Korean native cattle.
재료 및 방법
1. 공시축
2. 정자의 성 감별
3. 공란우의 과배란처리 및 인공수정
4. 수정란 회수 및 평가
5. 수정란 이식 및 임신진단
6. 전 혈액 배양으로부터 염색체 핵형 분석
7. 통계처리
결과 및 고찰
1. 수정란 회수
2. 발달단계별 수정란 회수
3. 수정란 이식 후 수태율
4. 성 감별 정자 유래 송아지의 염색체 핵형분석
REFERENCES
