The purpose of this study was to examine the effects of taurine and vitamin E on sperm characteristics damaged by bromopropane (BP) in pig. We evaluated toxicity of BP on viability, membrane integrity and mitochondrial activity of spermatozoa. 1-BP (0, 2.5, 5.0, 10, and 50 μM), 2-BP (0, 2.5, 5.0, 10, and 50 μM), taurine (0, 5.0, 10, and 25 μM) and vitamin E (0, 50, 100, and 200 μM) were treated in fresh boar semen for 6 h. 10 and 50 μM of 1-BP and 2-BP inhibited sperm viability, membrane integrity and mitochondrial activity in fresh boar semen (P<0.05). 25 μM of taurine increased sperm viability and membrane integrity (P<0.05), 100 μM of vitamin E enhanced viability and mitochondrial activity of sperm (P<0.05). Finally, 10 μM of 1-BP and 2-BP was co-treated with taurine (25 μM) and vitamin E (100 μM) in the fresh boar semen. The co-treated samples did affected viability, membrane integrity and mitochondrial activity of sperm. In conclusion, taurine and vitamin E can improve and maintain sperm quality in fresh boar semen.

INTRODUCTION
 MATERIALS AND METHODS
  1. Reagents and Sperm Preparation
  2. Sperm Viability
  3. Membrane Integrity
  4. Mitochondrial Activity
  5. Statistical Analysis
 RESULTS
  1. Bromopropane, 1-BP and 2-BP, Inhibits Sperm Characteristics inFresh Boar Semen
  2. Taurine Enhances Sperm Viability and Membrane Integrity
  3. Vitamin E Increases Viability and Mitochondrial Activity of Sperm
  4. Taurine and Vitamin E can protect Spermatozoa from BromopropaneDamage
 DISCUSSION
 REFERENCES

• Seunghyung Lee(College of Animal Life Sciences, Kangwon National University)
• Hee-Woo Park(College of Animal Life Sciences, Kangwon National University)
• Hee-Tae Cheong(School of Veterinary Medicine, Kangwon National University)
• Choon-Keun Park(College of Animal Life Sciences, Kangwon National University)
• Boo-Keun Yang(College of Animal Life Sciences, Kangwon National University) Corresponding author