The ω5-gliadins are the major allergens in wheat-dependent excise-induced anaphylaxis (WDEIA). In this study, SDS-PAGE analysis was used to assign the ω5-gliadins (Gli-B1) alleles in thirty two Korean wheat cultivars, compared with eleven standard wheat cultivars for Gli-B1a~m alleles. These results were reconfirmed with their complementary Glu-B3 low-molecular-weight glutenin subunits alleles tightly linked with Gli-B1 locus revealed with 2-DGE in our previous study. As a result, one Gli-B1b, four Gli-B1d, two Gli-B1f, six Gli-B1m and nineteen Gli-B1h varieties were identified. This is the first report on revealing the Gli-B1 alleles in Korean wheat cultivars and represents valuable basic data on wheat allergy, relationship between gliadin and wheat quality, and development of hypo-allergenic wheat.

서 언
 재료 및 방법
  재료
  글리아딘 추출
  SDS-PAGE 분석
 결과 및 고찰
  글리아딘 SDS-PAGE 분석에 의한 국내 밀 품종들의 Gli-B1 유전자좌 분석
 적요
 사사
 REFERENCES

• 장유란(국립농업과학원) | You-Ran Jang
• 범혜랑(국립농업과학원) | Hye-Rang Beom
• 김정봉(국립농업과학원) | Jung-Bong Kim
• 박철수(전북대학교 농업생명과학대학 작물생명과학과) | Chul-Soo Park
• 김영태(경상북도농업기술원 생물자원연구소) | Yeong-Tae Kim
• 강천식(국립식량과학원) | Chon-Sik Kang
• 임선형(국립농업과학원) | Sun-Hyung Lim
• 김영미(국립농업과학원) | Young-Mi Kim
• 이종렬(국립농업과학원) | Jong-Yeol Lee Corresponding author