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레스베라트롤 합성 GM 벼 검정을 위한 계통특이 마커 개발 KCI 등재

Development of Event-Specific PCR Marker for Identification of Transgenic Resveratrol-Enriched Rice Plant

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  • URLhttps://db.koreascholar.com/Article/Detail/316163
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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

A variety of genetically modified (GM) crops have been developed in Korea. In these crops, the resveratrol-enriched transgenic rice plant (Agb0102) has moved ahead to generate the dossier for regulatory review process required for commercialization of GM crop. The resveratrol-enriched transgenic rice plant could be released to farmers for cultivation after national regulators have determined that it is safe for the environment and human health. Here, we developed a PCR-based DNA marker based on flanking sequences of transgene for the discrimination of resveratrol-enriched transgenic rice plant. This DNA markers will be useful for identifying of resveratrol-enriched transgenic rice plant, and can also be used to estimate transgene movement occurred by pollen transfer or seed distribution. Moreover, it is helpful for prompt screening of a homozygote-transgenic progeny in the breeding program.

목차
서 언
 재료 및 방법
  식물재료
  DNA 추출
  계통 특이성 primer 제작 및 PCR 증폭 분석
 결과 및 고찰
  Agb0102 벼의 계통 특이 프라이머 제작
  PCR 증폭 방법을 이용한 Agb0102 벼 검출 조건 확립
  Agb0102 계통 특이 마커를 이용한 후대교배 계통 및 국내벼 품종 검정
 적 요
 사 사
 REFERENCES
저자
  • 우희종(농촌진흥청 국립농업과학원) | Hee-Jong Woo Corresponding author
  • 친양(농촌진흥청 국립농업과학원) | Yang Qin
  • 백소현(순천대학교 웰빙자원학과) | So-Hyeon Baek
  • 여윤수(농촌진흥청 국립농업과학원) | Yunsoo Yeo
  • 신공식(농촌진흥청 국립농업과학원) | Kong-Sik Shin
  • 임명호(농촌진흥청 국립농업과학원) | Myung-Ho Lim
  • 조현석(농촌진흥청 국립농업과학원) | Hyun-Suk Cho