This study was conducted to examine the effects of medium composition on organogenesis towards in-vitro cultured diploid and tetraploid Codonopsis lanceolata and obtain in-vitro mass propagation of superior species of C. lanceolata. Regarding MS medium composition for each concentration, diploid C. lanceolata was found to be declined. However, shoot and adventitious root formation were suppressed with higher mineral salt concentration, and active growth of shootand adventitious root was exhibited as 4.9 cm and 3.2 cm respectively in 1/2 MS medium. While in tetraploid C. lanceolata, it showed 2.9 cm and 3.2 cm respectively in 1/4 MS medium. In the case of sucrose concentration, no consistent decrease was observed for growth of shoot and adventitious root of diploid both at high and low concentration. The growth of shoot (at 3% concentration) and adventitious root (at 7% concentration) was 2.3 cm and 2.0 cm respectively. Although there was no difference in shoot formation of tetraploid C. lanceolata in all concentrations with the range of 1.7 ~ 1.8, there was a slight decrease in shoot growth at high concentration. Results revealed that the adventitious root formation was suppressed at high concentration. Concentration of agar exhibited no significant difference in shoot formation of diploid C. lanceolata at all concentrations. The highest result of adventitious growth (4.1 cm) was observed at 0.8% concentration. Slight inhibition of shoot formation and root formation of tetraploid C. lanceolata was observed at higher concentration. Shoot formation of diploid C. lanceolata also exhibited inhibition at higher concentration. Shoot formation of diploid C. lanceolata was increased at lower pH and shoot growth was the highest (2.3 cm) at pH 3.8. Adventitious root formation was higher at lower pH. Although there was no difference in shoot formation of tetraploid C. lanceolata presenting 1.7 ~ 1.8 regardless of high and low pH, growth inhibition was showed at higher pH. Adventitious root formation and growth showed a little higher result at pH 5.8.

MATERIALS & METHODS
 RESULTS & DISCUSSION
  Effects of various Concentration of culture mediumcompositions
  Sucrose Concentration
  Agar Concentration
  Effect of pH
 적 요
 REFERENCES

• Soo-Jeong Kwon(Dept. of Food Nutrition and Cookery, Woosong College, Daejeon 34606, Korea) | 권수정
• Dong-Yeon Seo(Dept. of Hotel and Restaurant Culinary Art, Kunjang University, Gunsan 54045, Korea) | 서동연
• Gab-Yeon Cho(Dept. of Food Science and Biotechnology, Woosong University, Daejeon 34606, Korea) | 조갑연
• Hyun-Jeong Kim(Dept. of Food Nutrition and Cookery, Woosong College, Daejeon 34606, Korea) | 김현정
• Moon-Soon Lee(Dept. of Industrial Plant Science & Technology, Chungbuk National University, Cheong-ju 28644, Korea) | 이문순
• Hee-Ock Boo(WELLPHYTO Co. Ltd., BI Center, GIST, Gwangju 61005, Korea) | (주)웰파이토
• Sun-Hee Woo(Dept. of Crop Science, Chungbuk National University, Cheong-ju 28644, Korea) | 우선희
• Hag Hyun Kim(Dept. of Food Nutrition and Cookery, Woosong College, Daejeon 34606, Korea) | 김학현 Corresponding author