Honeybees have faced many diseases which treaten bee colony including a serious population decline phenomenon called Colony Collapse Disorder (CCD). Nosema ceranae is a pathogen cause nosemosis, which is now wide spread around the world. According to the genome sequencing for N. ceranae, it has been identified that the presence of machinery for RNA silencing. Microsporidia N. ceranae that are obligated intracellular parasites depend on their host for energetic and metabolic needs. Here we selected the several genes from mitosome of N. ceranae to develop RNAi for the control of Nosema. Especially, TOM40, FNR1, FNR2 and Nar1 were chosen. After infection of N. ceranae, the Honeybee were treated with RNAi either by using only one or combining two or more. The infection rate and specific gene silecing in Nosema were analyzed.

• In Hui Kim(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Dong Jun Kim(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Hwi Geon Yun(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Won Seok Gwak(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Soo Dong Woo(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)