In 2015, we reported a viral disease extremely fatal to Allomyrina dichotoma larvae spread in the majority of the larva-rearing farms in Korea. Currently, the virus-infected larva is diagnosed by PCR-based amplification but this requires laboratory equipment and agarose gel electrophoresis. Loop-mediated isothermal amplification (LAMP), a highly sensitive DNA amplification method, uses DNA polymerase isolated from Bacillus stearothemophilus and a set of six primers. It has great potential for field use because DNA is amplified under single temperature and the overall reaction completes in 30 min without laboratory equipments. Here, we report the development of on-site diagnosis method for Korean horn beetle larva infected by Allomyrina dichotoma Nudivirus.

• Seokhyun Lee(Applied Entomology Division, National Academy of Agricultural Science, RDA)
• Ji-Young Choi(Applied Entomology Division, National Academy of Agricultural Science, RDA)
• Myung-Ha Song(Applied Entomology Division, National Academy of Agricultural Science, RDA)
• Kwan-Ho Park(Applied Entomology Division, National Academy of Agricultural Science, RDA)
• Kyu-Won Kwak(Applied Entomology Division, National Academy of Agricultural Science, RDA)
• Won-tae Kim(Applied Entomology Division, National Academy of Agricultural Science, RDA)