The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), is an economically damaging pest that feeds on most thin-skinned fruits. In this study, we sequenced portions of the mitochondrial (mt) COI and ND4 genes from a total of 195 individuals collected mainly from Korea. A total of 139 haplotypes were obtained from the concatenated COI and ND4 sequences. A dataset combining GenBank sequences with our own data identified a total of 94 worldwide COI haplotypes with a maximum sequence divergence of 5.433% (32 bp). A rough estimate of genetic diversity in each country showed higher diversity in ancestral distributional ranges, but the invasion over Asian countries seems to have been substantial because haplotype diversity was only 2.35-3.97-fold lower in the USA, Canada, and Italy than that in the populations ancestral ranges.

• Deuk-Soo Choi(Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon-si Gyeongsangbuk-do 39660 Republic of Korea)
• Jeong Sun Park(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)
• Min Jee Kim(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)
• Jong Seok Kim(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)
• Su Yeon Jeong(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)
• Jun Seong Jeong(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)
• Jinyoung Park(Division of Ecological Survey Research, National Institute of Ecology, Seocheon-gun, Chungcheongnam-do 33657, Republic of Korea)
• Iksoo Kim(College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea)