There are many other detection methods for Bursaphelenchus xylophilus. However, Baermann funnel method, PCR-based methods, which are laborious and time consuming processes that are unsuitable to rapid diagnose the Pine Wilt Disease (PWD) on the field. For these reasons, the aim of our experiment is not only to apply field diagnostic for pine wood nematode (PWN) but also to reduce total time for detection PWN in the pine trees by loop-mediated isothermal amplification (LAMP) with phosphate (Pi)-induced coloration reaction which could be yes or no answer for detection of PWN has not required UV detector but it just could be discriminated by naked eyes within 30 min. Genomic DNA was directly extracted from pine wood chips by Wood Chips Direct Lysis procedure which can be used for LAMP only after simple dilution. Our results suggest that LAMP-Pi detection method, simple and rapid method for detection of PWN, could be applied to the field diagnostic for PWD.

• Deok Jea Cha(Southern Forest Resources Research Center, National Institute of Forest Science)
• Dong Soo Kim(Southern Forest Resources Research Center, National Institute of Forest Science)
• Sang Gil Lee(Southern Forest Resources Research Center, National Institute of Forest Science)
• Si Hyeock Lee(School of Agricultural Biotechnology, Seoul National University)
• Yong Bae Park(Southern Forest Resources Research Center, National Institute of Forest Science)