The purpose of this study was to investigate alteration of chemical properties in red leaf lettuces(Lactuca sativa L.) exposed to digital music every day. The red leaf lettuces were cultivated in two hydroponic systems composed of two layers. In the first experiment, the red leaf lettuces with treatment were exposed to the digital music, while the lettuces under control condition were not exposed to the digital music. At harvest(6 weeks after planting), fresh weight and chlorophyll content were measured and compared the treatment with the control group. Subsequently, red leaf lettuces of the next experiment were compared to fresh weight, chlorophyll, ascorbic acid and anthocyanin content during different vegetation growth stages(4 weeks and 6 weeks after planting). The comparison of data for all experiment was also divided into upper and lower parts because of the difference of temperature in hydroponic systems. As a results, fresh weight and anthocyanin of the red leaf lettuces might be influenced by the difference of temperature variations. Chlorophyll of the red leaf lettuces was not easily influenced by digital music and difference of temperature. It was also shown that ascorbic acid as inactive molecule was not easily influenced by physical response like music.