Black soybeans, which have been widely utilized as food and as material for Oriental medicine, contain anthocyanins in the seed coat. Soybean seeds with black seed coat and green cotyledon are called “Seoritae” in Korea. Korean customer prefer “Seoritae” to normal black soybean with yellow cotyledon. The pigments contained in green cotyledon of black soybeans were chlorophylls and lutein. These constituents function as antioxidant and protect humans against diverse damages. The purpose of this study was to investigate the most suitable extraction condition and method of green cotyledon pigments in black soybeans. The green cotyledon pigments were analyzed on RP-HPLC with C18 column using gradient system. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 100% MeOH, and mobile phase B, consisting of 100% EtOAc. Among the 4 kinds of extraction method using methanol and/or acetone solution, ultrasonic extraction at 30 min. using acetone solution was most suitable extraction condition for simultaneous analysis of chlorophylls and lutein in soybean with green cotyledon.

• Young-Sun Hwang(Dept. of Herbal Medicine Resource, Kangwon National University)
• Hyeon-Jin Lee(Dept. of Herbal Medicine Resource, Kangwon National University)
• Su-San-Na Choi(Dept. of Herbal Medicine Resource, Kangwon National University)
• Su-Jin Lee(Dept. of Herbal Medicine Resource, Kangwon National University)
• In-Youl Baek(Yeongnam Agricultural Research Institute, NICS, RDA)
• Sung-Taeg Kang(National Institute of Crop Science, Rural Development Administration)
• Won-Young Han(Yeongnam Agricultural Research Institute, NICS, RDA)
• Jung-Kyung Moon(Yeongnam Agricultural Research Institute, NICS, RDA)
• Keum-Yong Park(Yeongnam Agricultural Research Institute, NICS, RDA)
• Myoung-Gun Choung(Dept. of Herbal Medicine Resource, Kangwon National University) Corresponding Author