Investigation of the plant growth affected by plug cell volume/pot size and plant age in herbaceous native plants belonging to 11 family and 24 species showed that growth of aerial part was different according to both plant age and plug cell volume/pot size. Among them, especially Iris dichotoma, Iris ensata var. spontanea, Iris pseudoacorus Lysimachia vulgaris var. davurica, Campanula glomerata var. dahurica and Solidago virgaurea subsp. asiatica showed more prominent difference depending on plug cell volume compared to other species. Regarding growth of subterranean part, as decrease plug cell volume/pot size root quality index became high while root weight became low, indicating that growth restriction was happened by exceed increase of root density due to limited root zone area. As increase cell volume/pot size, root development became active in bigger than 150 ㎖ of cell volume. Plant quality got better as increase cell volume/pot size, and 60 days old plants (DOP) revealed higher quality than 90 DOP revealed, indicating that marketing is possible from 60 DOP. Considering both plant age and cell volume (pot size), plant quality was better in smaller cell volume when cultivate plants in short time, while better in bigger cell volume when cultivate plants long time. Accordingly, when extend cultivation periods in small volume of plug cell, plant quality became bad. Therefore, this study showed that in order to produce high quality of plants, plants should be grown in proper plug cell volume depending on cultivation periods. Among plants tested Hosta longipes, Aquilegia buergeriana var. oxysepala, Aquilegia japonica, Veronica linariifolia, Hemerocallis fulva, Iris ensata var. spontanea, Iris pseudoacorus, Allium senescens, Hypericum ascyron, Caltha palustris var. palustris, Gentiana scabra, Campanula glomerata var. dahurica, Dendranthema boreale, Dendranthema zawadskii var. latilobum, Aster spathulifolius, Aster maackii, Aster koraiensis and Solidago virgaurea subsp. asiatica were determined to be able to produce standard size nursery plants using plug tray, and proper plug cell volume was bigger than 150 ㎖ (32 cell tray).