To select rice cultivars adaptable to low fertilization in puddled soil drill seeding, 20 rice cultivars were tested in Iksan. The seedling stand ratio of Kwanganbyeo, Junganbyeo, Saegyehwabyeo, Pyeonganbyeo were higher than 80%, but those of Hwalangbyeo, Hoanbyeo were lower than 60%. The tiller number per m2 was 4% smaller in low fertilization compared to conventional fertilization level. In low fertilization, the decrease rate of tiller number per m2 were less than 1~2% in Ansanbyeo, Juan 1, Geumanbyeo and Hoanbyeo, but more than 6~9% in Junganbyeo, Hwaanbyeo, Daesanbyeo and Saegyehwabyeo. Harvest index was 2.8% higher in low fertilization compared to conventional fertilization level. Nonganbyeo, Hopumbyeo, Dongjin 2 and Saegyehwabyeo showed high harvest index, but Gwanganbyeo, Geumanbyeo and Hwalangbyeo showed low harvest index. In low fertilization, milled rice protein content were lower than 6.0% in 14 rice cultivars including Juanbyeo and Kwanganbyeo, but 6.1~6.4% in other rice cultivars. Rice yield decreased 6% in low fertilization compared to conventional fertilization. Rice yield decrease were smaller as 2~3% in Hoanbyeo, Juanbyeo, Kwanganbyeo, Chengdambyeo, Geumanbyeo and Donganbyeo, but bigger as 7~8% in Nonganbyeo, Nonghobyeo. Dongjin 2, Saegyehwabyeo and Ansanbyeo. Owing to 2.4% increase of head rice ratio in low fertilization compared to conventional fertilization, head rice yield decreased 4% in low fertilization. Cultivating rice in puddled soil drill seeding with low fertilization, the rice cultivars such as Kwanganbyeo, Hopumbyeo, Pyeonganbyeo and Dong 2 showed lower than 6.0% milled rice protein content and more than 470㎏/10a milled rice yield.