A single-stage electrostatic precipitator (ESP) was evaluated in terms of its performance in removing dust in subway tunnels. A wire-to-plate type ESP was tested in a small-scale wind tunnel. The effects of wire-to-wire spacing (2040 mm) and the material connecting wire-to-wire on the performance of ESP were investigated, with varying applied voltage and airflow velocity. A narrower wire-to-wire spacing showed higher collection efficiency at the same applied voltage. Lower electrical resistivity of material connecting wire to wire was more effective. Ozone generation in subway tunnel applications was insignificant.