Anopheles sinensis is an important vector for Plasmodium vivax and thus has been targeted with pyrethroids in Republic of Korea. Using the direct contact mortality bioassay, two field populations of An. sinensis from Paju-si and Yeoncheon-gun were characterized for their resistance to pyrethroids with RR ratio values (Resistant ratio to imidacloprid) of 96.8 to 167.2 folds and 34.2 to 98.4 folds, respectively. Genomic DNA was used for Allele-specific PCR (AS-PCR) genotyping of the sodium channel genes to detect L1014S mutations. The homozygous susceptible Leu/Leu genotype in Paju-si and Yeoncheon-gun was 3.3% and 37.1% and the resistance genotypes were 96.7% and 62.9%, respectively. The homozygous Phe/Phe resistance genotype was the most prevalent as 45.0% in Paju-si and 31.5% in Yeoncheon-gun. Hence, this study suggests that malaria vector control programs should be prepared for the management of pyrethroid insecticide resistance.

• Chang-won Jang(Division of Medical Entomology, Center for Immunology and Pathology, NRIH, KCDC)
• Se-Jin Jeong(Division of Medical Entomology, Center for Immunology and Pathology, NRIH, KCDC)
• Young-Ran Ju(Division of Medical Entomology, Center for Immunology and Pathology, NRIH, KCDC)
• Kyu-Sik Chang(Division of Medical Entomology, Center for Immunology and Pathology, NRIH, KCDC)