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pp.287-295
1'he purpose 0 1' this study was to evaluate the ro1e of c• fos and c-jun expression in the salivary gland tumol‘ s , For this study‘ 11 s ubj ects of sali vary gland tumors ; 4 su이 ects of p1eomorphic adenomas, 3 s ubj ects of adenoid cystic car cinomas , 2 s ubjects of adenocar cinomas, 2 subjects of mucoepidermoid tumors, referred to the Dept, of Ora l Path College of Dentis t l'Y, Kyung Hee Univer sity, were used as experimental group, and 2 subj ect s of normal minor salivary gla nds without a ny infla mmator y changes, were used as control group respectively, All the tissues experimenta l and control group were fixed in 10% neutral formalin solution and embedded in paraffin , serial ti ssue section were made 5 I1I1l in thickness a nd processed in the s t andard way for immunohistochemical method, using primary and secondar y a ntibodi es, for c-fos, c-jun, foll owed by the Streptavidin-Horse Radish Peroxidase, all BioGenex U,S,A, made, appli cat ion counter s t ained with Mayer's hematoxylin stain method, mounted And examined under the biologic mi croscope, with the criteria : -(no epitheli al s ta in), +(weak or focal epithelial stain), ++(moder a te or focal intensive epithelial sta in)‘ +++(intense generali zed epithelial staining) for the epithelial, and stromal ti ssues on each Attained results as follows : 1 1n nonna l minor saliva ry gla nds , it is noted that negative responses on the acini minimal res ponses in nucl ei and cytoplams of se rous demilun e, myoepi theli al cells, and intensive r esponses in nuclei and cytoplam of ducta l cells to c-fos and c-jun, 2 1n the res ponses to c-fos, positive responses in nuclei and cytoplasms of the lining cells o[ the ad e nαd tissues, e pidermoid, and mucous cells in mucoepidermoid tumors are noted and in other tumor tissuses, negative nuclei wi th pos itive cyto plasms are revealed 3 1n the responses to c- jun, it is noted that positive r es ponse in nuclei and cytolasm i n the cells of adenoid tissues in pl eomorphic adenoma, epidermoid cell s, mucous cell in mucoe pi dermoid tumor but in other tumor s, only positive responses in cytoplasm are noted, Intensive r esponses on c-fos‘ c-jun were noted on the high a typical cells 1'his results suggest that c-fos and c-jun may be affected t o the reactivation on growt h and development of the salivary gland tumors
