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pp.271-282
The aim of this study was to investigate the cytotoxicity of dental casting gold alloys. Recently, "biocompatability" is considered the most important requirement of dental materials. Dental metals and alloys were estimated by quantity of released ions, which had influenced to living tissues. The requirement of using normal human cells for cytoxicity strudy were abruptly increased. We used the cultured normal human gingival fibroblasts to estimate the cytotoxicity of dental casting gold alloys. The product of S company(Korea, AIGIS-SOFT, AIGIS-PLUS, AIGIS-A, AIGIS-PT, experimental group) and D company's (German, Biocclus inlay, Biolor SG, Stabilor NF Ⅳ, Degulor B, control group) dental casting gold alloys were used. The morphological investigation, hemolysis test, MTT assay and SRB assay were done in vitro. In vivo, inflammatory reaction in rat was examined for 2 weeks. 1. In the result of cytotoxicity assay, there were some differences but was no significancy among the results between two group's hemolysis, MTT and SRB assay. 2. The gingival fibroblasts attached to the surface of dental casting gold alloy showed various features and increased in number as the time had passed. 3. In vivo, chronic inflammatory cell infiltration was prominent from 3 days to 1 week and inflammation was reduced as time had gone. From the aboving results, there were no significant differences in cytoxicity depending on the ratio of gold content, but showed differences depending on the ratio of total precious and non-precious metal content between two groups. In vitro study showed few differences in inflamation reaction.
