The aim of this study was to investigate the cytotoxicity of dental casting gold alloys. Recently, "biocompatability" is considered the most important requirement of dental materials. Dental metals and alloys were estimated by quantity of released ions, which had influenced to living tissues. The requirement of using normal human cells for cytoxicity strudy were abruptly increased. We used the cultured normal human gingival fibroblasts to estimate the cytotoxicity of dental casting gold alloys. The product of S company(Korea, AIGIS-SOFT, AIGIS-PLUS, AIGIS-A, AIGIS-PT, experimental group) and D company's (German, Biocclus inlay, Biolor SG, Stabilor NF Ⅳ, Degulor B, control group) dental casting gold alloys were used. The morphological investigation, hemolysis test, MTT assay and SRB assay were done in vitro. In vivo, inflammatory reaction in rat was examined for 2 weeks. 1. In the result of cytotoxicity assay, there were some differences but was no significancy among the results between two group's hemolysis, MTT and SRB assay. 2. The gingival fibroblasts attached to the surface of dental casting gold alloy showed various features and increased in number as the time had passed. 3. In vivo, chronic inflammatory cell infiltration was prominent from 3 days to 1 week and inflammation was reduced as time had gone. From the aboving results, there were no significant differences in cytoxicity depending on the ratio of gold content, but showed differences depending on the ratio of total precious and non-precious metal content between two groups. In vitro study showed few differences in inflamation reaction.
This paper present a novel approach to control the lower body power assistive exoskeleton system of a HEXAR-CR35 aimed at improving a muscular strength. More specifically the control of based on the human intention is crucial of importance to ensure intuitive and dexterous motion with the human. In this contribution, we proposed the detection algorithm of the human intention using the MCRS which are developed to measure the contraction of the muscle with variation of the circumference. The proposed algorithm provides a joint motion of exoskeleton corresponding the relate muscles. The main advantages of the algorithm are its simplicity, computational efficiency to control one joint of the HEXAR-CR35 which are consisted knee-active type exoskeleton (the other joints are consisted with the passive or quasi-passive joints that can be arranged by analyzing of the human joint functions). As a consequence, the motion of exoskeleton is generated according to the gait phase: swing and stance phase which are determined by the foot insole sensors. The experimental evaluation of the proposed algorithm is achieved in walking with the exoskeleton while carrying the external mass in the back side.
In this paper, four-bar linkage mechanism for the knee joint is developed which is used in prosthetics. But unlike the prosthetics, the feature of this mechanism is that the instantaneous center of rotation of the four-bar linkages can be moved behind the ground reaction force vector so that it can be passively supported without any external power. In addition, this mechanism is developed similar to the structure of the human knee joint for eliminating the sense of heterogeneity of the wearer. In order to design the mechanism with these two objectives, optimization design process is done using the PIAnO tool and detailed design is carried out through optimized variable values. The developed mechanism is attached to the robot which can assist the hip and ankle joints. In order to verify the operation of the developed knee mechanism, an insole type sensor was attached to the shoes to compare data values before and after wearing the robot. Result data showed that wearer wearing the exoskeleton robot with the knee mechanism was the same value regardless of whether the heavy tool is loaded or not.
Twenty four Holstein steers (average body weight 714 ± 13.60 kg) were used in this experiment to determine the effect of supplementing of microbial culture and coated vitamin-C on growth performances and carcass characteristics in finishing Holstein steers. Holstein steers were randomly assigned to feeding groups of control group (Con, 12 kg of basal diet/head/day), microbial culture group (MC, 12 kg of basal diet + 30 g of microbial culture/head/day) and coated vitamin-C group (CVC, 12 kg of basal diet + 10 g of coated vitamin-C/head/day). MC and CVC groups were higher in ADG compared to control (P<0.05). FCR was also lower in MC and CVC groups than control group (P<0.05). Back fat thickness, rib-eye area, marbling score, meat color and yield index were not changed by supplementing microbial culture and coated vitamin-C. MC group was higher for maturity compared to control and CVC group (P<0.05). CVC group was higher for fat color compared to control and MC group (P<0.05). Based on the results obtained from the current study, supplementation of microbial culture and coated vitamin-C as an alternative to antibiotic might increase growth performances and enhance carcass characteristics in finishing Holstein steers. However, more studies are needed to find out the optimum supplementing period of microbial culture or coated vitamin-C for high quality meat production from Holstein steers.