논문 상세보기
pp.10-15
Background: We investigated the antioxidative and tyrosinase inhibitory activities of 70% ethanol extract, and its fractions, of the root of Rumex japonicus HOUTT.
Methods and Results: The total phenolic compound contents of the 70% ethanol extract and ethyl acetate fraction were 168.99㎎/ g and 651.78㎎/g, respectively. The antioxidant activity was compared through the DPPH radical and nitric oxide (NO) scavenging assays. The ethyl acetate fraction showed the highest DPPH radical and NO scavenging abilities, which confirmed the antioxidant activity. Specifically, the ethyl acetate fraction showed a higher DPPH radical scavenging ability than ascorbic acid. These results were related to the total phenolic compound content of the ethyl acetate fraction. Moreover, in the tyrosinase inhibition assay, the ethyl acetate fraction exhibited stronger inhibitory activity than arbutin, which was used as the positive control. The cell viability of L929 cells was analyzed by MTT assay after treatment with 70% ethanol extract and all fractions; no changes in viability were observed, which demonstrated the nontoxic nature of the extract and fractions. Conclusions: These results suggested that the extract from the root of R. japonicus and its ethyl acetate fraction could be a novel resource for the development of a cosmetic with antioxidant and tyrosinase inhibitory activity.
재료 및 방법
1. 재료
2. 추출 및 분획
3. 사용 균주와 세포주 및 시약
4. 총 phenol성 화합물 함량 측정
5. DPPH radical 소거능 측정
6. Nitric oxide 소거능 측정
7. Tyrosinase 저해 활성 측정
8. 세포 생존율 측정
9. 통계분석
결과 및 고찰
1. 총 phenol성 화합물 함량
2. DPPH radical 소거능
3. NO 소거능
4. Tyrosinase 저해 활성
5. MTT assay에 의한 세포독성
REFERENCES
