Phenolics from the Twig of Broussonetia kazinoki
Background : Phenolic compounds were isolated from the twig of Broussoneita Kazinoki. Their structures were established on the basis of extensive spectroscopic (MS, 1D , and 2D NMR) data analysis and by comparison with the spectroscopic data reported in the literature. Methods and Results : The twig of B. Kazinoki were extracted 60% aqueous ethanol for 3 days at room temperature. The extract was filtered and concentrated by vacuum evaporator. And then, extract was partitioned using hexane, methylene chloride (MC), ethyl acetate (EtOAc), n-butyl alcohol (BuOH) and H2O, successively. The extraction was separated by using prep-HPLC, and the structure was analyzed by Mass spectrometry (MS) and 1H-, 13C-, 1H-1H COSY, HSQC, HMBC NMR data. Conclusion : These compounds were identified as chlorogenic acid (1), ferulic acid (2), p-coumaric acid (3), taxifolin (4), marmesin (5), 5-methoxy marmesin (6), pinoresinol (7), syringaresinol (8), quercetin (9), broussonin A (10), broussonin B (11), broussoflavonol A (12), broussoflavonol B (13), kazinol A (14), and 5,7,3',4'-tetrahydroxy-3-methoxy-8,5'-diprenylflavone (15).