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In vitro에서 조릿대 잎 추출물의 생리학적 효능
Effect of Sasa quelpaertensis Nakai Leaf Extract on Bioavailability Assay In vitro
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/326428
Background : Indigenous plant in Jeju island, Sasa quelpaertensis Nakai, belongs to the Bambusoideae and inhabit around Mt. Halla. According to the ancient book such as Dongui Bogam, Sasa quelpaertensis Nakai have been known to possess the antidiabetic, anti-inflammatory, and anti-diuresis effect. However, because of gradual upturning temperature, Sasa quelpaertensis Nakai was spread out to wider area and intrude the habitat that other plant species are growing. Recently, although the study to seek effective use of Sasa quelpaertensis Nakai, the investigation about functional properties has not been taken place enough. Methods and Results : To assess the inhibitory activity of melanin synthesis, we employ the tyrosine as substrate and measure the formation of dopaquione at 490 nm. Firstly, 0.1 mM potassium phosphate buffer and tyrosinase were mixed and incubated at 37℃. After incubating at 37℃, the absorbance rate was measured at 490 nm. The value was compared with positive control, arbutin, and calculated with the rate between sample and control value. Previously, formononetin, glabrene, glabridin, glabrol, artocarbene, dihydromoriin are known as effective substances for whitening. Moreover, the arbutin, which was separated from Arctostaphylos uva-ursi (L.) Sprengel, are widely used in cosmetic field. Arbutin inhibits tyrosinase and tyrosine synthesis, which induce blackish pigmentation. Practically, the Sasa quelpaertensis Nakai leaf ethanol extract depend on different solvent condition, whole extracts showed stronger inhibition than arbutin. Especially, 60% ethanol extract exhibited twice higher tyrosinase inhibitory activity than arbutin, whereas least inhibitory activity was seen in 20% ethanol extract. Conclusion : In this study, a attempt was made to investigate the tyrosinase inhibitory activity of Sasa quelpaertensis Nakai leaves extracted by different solvent condition. In the results, each extracts was prior to arbutin. Yet, 20% ethanol extract was lowest, but on the one hand, 60% ethanol extract demonstrated the highest tyrosinase inhibitory activity.
