Background : Platycodon grandiflorum is a perennial plant and a member of Camanulaceae family. Since ancient times, they have been using P. grandiflorum as an important medicinal plant in Korea. Platycodin D is the most abundant saponin derived from P. grandiflorum and pharmacologically active component. UDP-glycosyltransferases (UGTs) are important enzymes in the saponin biosynthesis. UGT is a glycosyltransferase and act on the final step of the secondary metabolite biosynthesis. Methods and Results : We tried to identify UGT genes related to saponin biosynthesis of P. grandiflorum through RNA-seq analysis. The sequencing was performed using Illumina Hi-Seq platform after cDNA library preparation. The produced reads were assembled using CLC Genomics Workbench software (CLC Bio, Inc.). We obtained 122,663 contigs and found 137 putative UGT genes. Familes of UGT71, UGT73, and UGT74 were selected as putative saponin biosynthesis related gene families using phylogenetic relationship analysis. qPCR condition about UGT73 is preheating 94℃ 180 sec, denaturation 94℃ 60 sec, annealing 53℃ 60 sec, extension 72℃ 90 sec, final extension 72℃ 600 sec, 45 cycles repeated. Conclusion : The results in this study could help to find the UGTs related to saponin biosynthesis pathway of P. grandiflorum.

• Sohyeon Park(Department of Industrial Plant Science and Technology, Chungbuk National University) | 박소현
• Jemin Yoo(Department of Industrial Plant Science and Technology, Chungbuk National University) | 유제민
• Yurry Um(Department of Herbal Crop Research, NIHHS, RDA) | 엄유리
• Ok Tae Kim(Department of Herbal Crop Research, NIHHS, RDA) | 김옥태
• Chang Pyo Hong(TheragenEtex Bio Institute, Theragen Etex) | 홍창표
• Seong Cheol Kim(Department of Herbal Crop Research, NIHHS, RDA) | 김성철
• Yi Lee(Department of Industrial Plant Science and Technology, Chungbuk National University) | 이 이 Corresponding author