Background : Tetraploid plant is bigger in size of organs like leaf, stem, fruit, and root than diploid plant by chromosome doubling. Also, Self-ìncompatibílity disappeares and disease resistance is strengthened in tetraploid plant. To breed new boxthorn variety having bigger fruit, higher yield, pest tolerance, and, self-compatibílity, tetraploids was induced for tetraploid breeding resources by colchicine treatment in each boxthorn variety. Materials and Methods : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Polyploid was identified by flow cytomertry(Partec, PA-1) and chromosome numbers of polyploid individuals were examined by aceto-carmine stain. Conclusion : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Tetraploid was induced as 13, 9, 6, and 5 individuals in Myongan, Cheongdae, Bulro, and Youngha respectively by above colchicine treatment.

• Su Dong Kim(Ginseng and Medicinal Plant Research Institute, CNARES) | 김수동 Corresponding author
• Seung Ho Han(Ginseng and Medicinal Plant Research Institute, CNARES) | 한승호
• Hyun Ho Kim(Ginseng and Medicinal Plant Research Institute, CNARES) | 김현호
• Young Chun Park(Cheongyang Boxthorn Experiment Station Ginseng and Medicinal Plant Research Institute, CNARES) | 박영춘
• Sang Nag Ahn(College of Agriculture and Life Science, Chungnam National University) | 안상락