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리그닌 생합성 관련 유전자 COMT를 이용한 참억새 형질전환 체계 확립
Establishment of Miscanthus Sinensis Transformed by Agrobacterium Using COMT Gene Related to Lignin Biosynthesis
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/326449
Background : Miscanthus is a diploid hybrid and a temperate, perennial, cross-pollinating grass used as bioenergy plant, biomass production and high quality cellulose and ethanol production. This study was to determine an efficient transformation system for Miscanthus sinensis, and to optimize factors and conditions required for expression of MsCOMT–AS gene. Methods and Results : An efficient transformation of callus from M. sinensis was established using Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pMBP1. In order to establish stable transformation system, we obtained high transformation rate from callus by various transformation factor explant type, strain, co-culture periods, acetosyringone concentration, and selective marker. Finally, in this study, seven putative transgenic plants were obtained. Through various tests including PCR analysis and southern blot were to detect antisense of COMT digested Xba I and Sac I restriction enzymes. The biomass of the control plant was superior than transgenic plants. Conclusion : This study was to develop transgenic Miscanthus sinensis by Agrobacterium tumerfeciens mediated transformation to produce high bioethanols and to reduce the lignin content of transgenic plants. Detailed characterization of the transgenic plants revealed interesting finding about COMT gene expression in the segregates
