Background : barley (Hordeum vulgare L.), is a member of the grass family Poaceae (Gramineae) consider the fourth most important cereal crop and primarily used to feed the animals. The second most common use of barely is for malt, which is used mostly in beer, but also in hard liquor, malted milk and flavoring in a variety of foods. As immense economic importance a fast and high germination is required in order to achieve its desired production. In regard to this the present study was performed to evaluate the effect of beneficial microbes (BM), on barely seed germination when mixed with in the synthetic germination medium. Methods and Results : Barely seeds were surface sterilized using 0.01% HgCl2 solution and transfer for germination in the medium supplemented with 200 and 500 X diluted BM separately. Effectiveness of the BM were determined by calculating the germination percentage (GP), germination index (GI), mean germination time (MGT), germination time 50% (T50) and compared with control seeds germinating in absence of BM. Results obtained from the study suggest that BM at 200 X dilution is much efficient to speed up the germination process (66.7 ± 2.3 %) in contrast to 500X diluted BM (61.4 ± 3.6 %). Results of the other germination parameters (GI, MGT and T50) supported the 200X BM effective for seed germination as compare to 500X and control. Conclusion : Our results signify that BM has potential to enhance the germination efficacy of barley seeds and thus could be used to improve the germination.

• Sun Chul Kang(Department of Biotechnology, Daegu University) | 강선철 Corresponding author
• Ashutosh Bahuguna(Department of Biotechnology, Daegu University) | 아쉬토쉬 바후구나