Background : Oxidative stress condition refers as disturbed homeostasis between the generation of reactive oxygen species (ROS) and the antioxidant system that results in severe pathological conditions. To balance such an oxidative stress condition external antioxidant sources are required. Methods and Results : In view of the above, present study was carried out to investigate the cytoprotective role of the strawberry (Fragaria × ananassa var. Seolhyang) root extract (SRE) against oxidative stress condition stimulated by H2O2 in murine macrophages Raw 264.7 cells and further evaluation of intracellular ROS level, lipid peroxidation and myeloperoxidase (MPO) activity as oxidative stress markers. Cytoprotective effect of SRE was evaluated by quantifying survival of macrophages challenged by H2O2 in presence and absence of SRE. Finding suggest a 60.1 ± 7.5% cell survival in H2O2 treated cells as compare to 100% survival in control cells. However, a significant increase in cell survival with 62.7 ± 2.9, 74.2 ± 5.2 and 81.3 ± 3.3% were observed in the cells treated with 2.5, 5, 10 μg/ml of SRE respectively. A reduced ROS level together with inhibition of the lipid peroxidation and MPO activity was observed in the cells treated with SRE as compare to only H2O2 treated cells. Conclusion : Our results signify the potency of SRE to protect the cells from oxidative stress implied by H2O2 and can be used as a source of antioxidant for health benefits.

• Ashutosh Bahuguna(Department of Biotechnology, Daegu University) | 아쉬토쉬 바후구나
• Sun Chul Kang(Department of Biotechnology, Daegu University) | 강선철 Corresponding author