Background : Ginseng has been commonly used as a traditional oriental medicine for its wide spectrum of medicinal properties, including anti-inflammatory, antitumorigenic, adaptogenic and anti-aging properties. 20(S)-Protopanaxadiol (PPD), a intestinal metabolite of ginsenosides, is one of the active ingredients in ginseng. In this study, we have found inflammation-related genes regulated by 20(S)-PPD in mouse bone marrow-derived macrophage (BMDM) to elucidate the role of 20(S)-PPD in inflammatory signaling pathways. Methods and Results : We examined cell viability of BMDM cells after treatment of 20(S)-PPD and found that 20(S)-PPD has no cytotoxicity up to 10 uM. BMDM cells treated with none or 10uM 20(S)-PPD were used for RNA extraction and microarray analysis. It was found that 2 inflammation-related genes are upregulated and 4 genes are downregulated by 20(S)-PPD. Conclusion : These results can give clues to elucidate the role of 20(S)-PPD in inflammatory signaling pathways.

• Ji Hye Yoon(Department of Herbal Crop Research, RDA) | 윤지혜
• Chun Geon Park(Department of Herbal Crop Research, RDA) | 박춘근
• Young Sup Ahn(Department of Herbal Crop Research, RDA) | 안영섭
• Jehun Choi(Department of Herbal Crop Research, RDA) | 최재훈
• Young Seob Lee(Department of Herbal Crop Research, RDA) | 이영섭
• Doo Jin Choi(Department of Herbal Crop Research, RDA) | 최두진
• Hyung Don Kim(Department of Herbal Crop Research, RDA) | 김형돈 Corresponding author