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참억새로부터 분리된 S-Adenosylmethionine Synthetase (SAMS) 유전자를 이용한 담배 형질전환체계 확립

Establishment of Transgenic Nicotiana benthamiana by using S-Adenosylmethionine Synthetase (SAMS) Gene Isolated from Miscanthus sinensis

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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : Temperature is major factor for growth plant. Recently, because of global warming, abnormal temperature included drought, deluge, sudden temperature change and heavy snow damaged crops in the world. In Korea, crops have been sensitive to low temperature on early growth stage, e.g. fruit tree and ginseng, were damaged owing to sudden heavy snow and cold on Spring. Therefore, recently interest in cold resistance crops were increased in demand rapidly. This study was performed to establish transgenic Nicotiana benthamiana by transforming cold resistant gene related to cold tolerance S-adenosylmethionine synthetase (SAMS) isolated from Miscanthus sinensis. Methods and Results : Total RNA was extracted from leaves of M. sinensis using Trizol assay and isolated MsSAMS. Isolated MsSAMS was insert into SacⅠ- XbaⅠ sites of pMBP1 vector. The vector was transformed to Agrobacterium tumefaciens strain LBA4404 by DH5α. A. tumefaciens with binary plasmid were selected at YEP medium supplemented with kanamycin. Cut leaves of tobacco were co-cultured with selected A. tumefaciens. Co-cultured leaves was grown on regeneration medium for a month at dark condition, and transferred to at light condition. Regeneration shoot from callus were excised and transferred to root-induction medium. Approximately, 58% of leaves explant produced callus. Nearly, 30% of callus had shoot and approximately, 94% of shoots were rooted in root-induction medium. Conclusion : We established an efficient transformation system of N. benthamiana transformed by using MsSAMS gene related to cold tolerance isolated from M. sinensis. We may use the produced transgenic plants to prevent damages carried by cold.

저자
  • Mi Ran Jeon(Department of Bio-Resource Sciences, Kangwon National University) | 전미란
  • Eun Soo Seong(Department of Medicinal Plant, Suwon Women’s University) | 성은수
  • Ji Hye Yoo(Bioherb Research Institute, Kangwon National University) | 유지혜
  • Jae Hoo Choi(Department of Bio-Resource Sciences, Kangwon National University) | 최재후
  • Chang Heum Kim(Department of Bio-Resource Sciences, Kangwon National University) | 김창흠
  • Byeong Ju Kang(Department of Bio-Resource Sciences, Kangwon National University) | 강병주
  • Kweon Heo(Department of Bio-Resource Sciences, Kangwon National University) | 허권
  • Chang Yeon Yu(Department of Bio-Resource Sciences, Kangwon National University) | 유창연 Corresponding author