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들깨 종자의 GA3 발아 증진 효과 및 발아 관련 유전자 발현양상 검정

Effects of GA3 on Seed Germination Rates and Expression of Germination-Related Genes in Perilla

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  • URLhttps://db.koreascholar.com/Article/Detail/326717
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : Perilla frutescens L. is a bisexual, annual plant belonging to the Labiatae. Dormancy period of Perilla seed usually ranged from 85-200 days. The germination enhancing effects in a reduced seed vigor during the GA3 treatment has been reported. Objectives of the present study was to evaluate the effect of GA3 on germination rate and the expression of germination-related genes expression by using perilla seeds. Methods and Results : Three different cultivars (Saeyeopsil, Okdong) and accession (line 141) of perilla were used in the experiment and treated with GA3 at different concentration (50, 100, 300, 500 μM) for one day at dark condition. Germination test for perilla seed was carried out by using 100 perilla seeds treated with GA3 at 25℃. Rate of germination were evaluated after 10 days and the experiments were repeated three times in similar condition. Samples were collected from each cultivar and accessions for RNA extraction. After cDNA synthesis quantification, templates were subjected to RT-PCR using actin primers. EST blast performed for sequencing the RT-PCR products. Conclusion : Result showed that 100μM GA3 treated seeds of three perilla cultivars and accessions showed the highest germination rate. However, concentration of GA3 over 100 μM and lower than 100 μM resulted into reduced germination rate. Furthermore, expression of germination-related genes from 100 μM treated GA3 was higher compared to untreated sample by using 100 μM GA3 treated sample by using pC12, pJ14 primers.

저자
  • Byeong Ju Kang(Department of Bio-Resource Sciences, Kangwon National University) | 강병주
  • Ji Hye Yoo(Bioherb Research Institute, Kangwon National University) | 유지혜
  • Jae Hoo Choi(Department of Bio-Resource Sciences, Kangwon National University) | 최재후
  • Chang Heum Kim(Department of Bio-Resource Sciences, Kangwon National University) | 김창흠
  • Mi Ran Jeon(Department of Bio-Resource Sciences, Kangwon National University) | 전미란
  • Eun Soo Seong(Department of Medicinal Plant, Suwon Women’s University) | 성은수
  • Yea Ji Lee(Research Institute of Biotechnology, Hwajin Cosmetics) | 이예지
  • Jae Geun Lee(Research Institute of Biotechnology, Hwajin Cosmetics) | 이재근
  • Chang Yeon Yu(Department of Bio-Resource Sciences, Kangwon National University Bioherb Research Institute, Kangwon National University) | 유창연 Corresponding author