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당귀 종판별을 위한 엽록체 기반 SSR 마커 개발

Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation

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  • URLhttps://db.koreascholar.com/Article/Detail/326721
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species. Methods and Results : A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00. Conclusions : These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.

저자
  • Sang Ik Park(Department of Industrial Plant Science and Technology, Chungbuk National University) | 박상익
  • Serim Kim(Department of Industrial Plant Science and Technology, Chungbuk National University) | 김세림
  • Jinsu Gil(Department of Industrial Plant Science and Technology, Chungbuk National University) | 길진수
  • Yi Lee(Department of Industrial Plant Science and Technology, Chungbuk National University) | 이이
  • Ho Bang Kim(Life Sciences Research Institute, Biomedic Co. Ltd.) | 김호방
  • Jungho Lee(Green Plant Institute) | 이정호
  • Seong Cheol Kim(Department of Herbal Crop Research, NIHHS, RDA) | 김성철
  • Chan Sik Jung(Department of Herbal Crop Research, NIHHS, RDA) | 정찬식
  • Yunmi Park(Forest Medicinal Resources Research Center, National Institute of Forest Science) | 박윤미
  • Kwon Seok Jeon(Forest Medicinal Resources Research Center, National Institute of Forest Science) | 전권석
  • Yurry Um(Forest Medicinal Resources Research Center, National Institute of Forest Science) | 엄유리 Corresponding author