To estimate pollution sources in the watershed with various industries, the simulation of contamination route and distribution of 2-methyl-4,6-dinitriophenol(DNOC) were performed with a numerical model Hydro Geo Sphere. This study was performed calculations of the load using the measured concentration and simulated flow rate. And, the river was divided by the sampling sites at the mainstream, and the contribution rate at downstream sampling sites was calculated for each section. The results showed the concentration of the downstream sampling sites were decided by the concentration of upstream sites, and the contribution rates of the tributaries were calculated below 10%. The results also showed that the impact of the potential sources in Section 1(Geumho1 ~ Geumho2) and Section 5(Geumho5 ~ Geumho6) was larger than in the other area. In Section1 and Section5, It seemed to require detailed investigation.