The feeding habits of the Pacific bluefin tuna, Thunnus orientalis were examined based on the stomach contents of 330 specimens caught by offshore large purse seine fishery in the southern sea of Korea, 2011. The size of Pacific bluefin tuna used in this study ranged from 34.1 to 67.3 cm in fork length (FL). The percentage of empty stomachs was 41.8%. The main prey items were Pisces and Cephalopoda based on percentage IRI (index of relative importance). The main fish preys were Trachurus japonicus, Bregmaceros japonicus, Engraulis japonicus, and Cephalopoda were Todarodes pacificus, Loligo edulis, Watasenia scintillans. T. orientalis showed ontogenetic change in diet composition. Although Pisces dominated the diet of all length classes the portion of Cephalopoda was relatively higher in size between 40 and 50 cm other than length classes. In terms of seasonal variation in feeding habits, Pisces was the main prey group in all seasons, but Cephalopoda was also frequently consumed during spring and autumn based on %IRI.