In order to evaluate CMV resistance of the radish inbred lines, leaves of twenties radish (Raphanus sativus L.) inbred lines were mechanically inoculated with a strain of Cucumber mosaic virus (CMV) systematically infecting radish plants, respectively. The inoculated radish plants were incubated at 22°C±3°C and resistance assessment was examined using symptom development for 4 weeks. Based on the reactions of differential radish inbred lines, 11 radish lines were produced mosaic, mottling, yellowing and severe mosaic symptoms by CMV infection. These results were confirmed by RT-PCR analysis of CMV coat protein gene, suggesting that CMV is responsible for the disease symptoms. In contrast, 9 resistant radish lines did not induce systemic mosaic symptoms on upper leaves and chlorosis in stem tissues for 4 weeks, showing that the radish plants did not express any symptoms by 8 weeks. Further examination of CMV infection in the 9 resistance-candidate radish lines showed no CMV infection in systemic leaves of the 9 radish lines. Taken together, these results suggest that the 9 radish lines are highly resistant to CMV.

• 윤주연(국립원예특작과학원 원예특작환경과) | Ju-Yeon Yoon (Department of Horticultural and Herbal Environment, National Institute of Horticultural and Herbal Science, Wanju, 55365, Korea)
• 최국선(국립원예특작과학원 원예특작환경과) | Gug-Seoun Choi (Department of Horticultural and Herbal Environment, National Institute of Horticultural and Herbal Science, Wanju, 55365, Korea)
• 김수(국립원예특작과학원 채소과) | Su Kim (Department of Vegetables, National Institute of Horticultural and Herbal Science, Wanju, 55365, Korea)
• 최승국(국립원예특작과학원 채소과) | Seung-Kook Choi (Department of Vegetables, National Institute of Horticultural and Herbal Science, Wanju, 55365, Korea) Corresponding author