The activities of three class, five acaricides (Permethrin, Deltamethrin, Fenitrothion, Chlorpyrifos, Imidacloprid) againstHaemaphysalis longicornis nymph, a major vector for Severe Fever with Thrombo cytopenia Syndrome (SFTS) and Lymedisease. The ticks distribute at vast open fields and various kind of habitats. Five acaricides were applied to ticks collectedform four areas(Gapyeong, Paju, Chuncheon, Wonju) to confirm the effect of acaricide. This areas was the area whereSFTS disease occurred and a large nember of ticks were collected. As a result, All areas is the most acaricidal effectto Deltamethrin. And two areas(Gapyeong, Paju) has the lowest acaricidal effect to Imidacloprid and two area(Chuncheon,Wonju) has the lowest acaricidal effect to Permethrin. Futher study will need to compare the acaricidal efficacy of theremaining seven province.