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Monitoring of organophosphate and pyrethroid resistance of Aedes albopictus from two areas in the Republic of Korea (ROK), 2017
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- URLhttps://db.koreascholar.com/Article/Detail/334379
Aedes albopictus is an important vector for yellow fever, dengue fever, chikungunya fever and Zika virus. This mosquitohas been exposed to organophosphates and pyrethroids in Republic of Korea for a long time. Using the direct contactmortality bioassay, susceptibility strain and two field populations of Ae. albopictus from Busan, and Damyang. Insecticidalproperty were indicated to RR ratio values (Resistant ratio to strain of susceptibility) of LC50 (Midian Lethal concentration)to organophosphats and pyrethroids. Genomic DNA was used for Allele-specific PCR (AS-PCR) genotyping of the acethylcholineesterase to detect Gly119Ser mutations and kdr gene to detect Phe1534Cys mutations. Detoxification enzyme activitiesof Ae. albopictus from Busan and Damyang were assessed using microplate enzyme activity assays. Activities of fourdetoxification enzymes eg., glutathione S-transferase(GST), Non specific esterases (α-naphthyl acetate and β-naphthyl acetate),and cytochrome C oxidase were determined for each Ae. albopictus strain. This study might suggest that Ae. albopictuscontrol programs should be prepared for the management of organophosphate and pyrethroid insecticide resistance.
