The exosomes are the most studied nanometer-sized extracellular vesicles (40-100 nm) in eukaryotic cell. Exosomeharbors various molecular components of their cell origin, including nucleic acids and proteins, and it is involved in intraand intercellular communication. These characteristics make exosomes to be a prospective biomarkers, therapeutic agents,or drug delivery vehicles. Edible insect industry is rapidly growing up in Korea. The insect exosomes were isolated fromthe larvae of Korean rhinoceros beetle, Allomyrina dichotoma, and White-spotted flower chafer, Protaetia brevitarsis, sothat they can be used for diagnosis of insect disease. The stable preservation of exosome is very important for diagnosisand research, especially for long term storage. Here, the stable recovery of exosome isolated from hemolymph of A. dichotomalarva was evaluated by analyzing exosomal protein and RNA after storage in -70°C for three months.

• Seokhyun Lee(Applied Entomology Division, National Institute of Agricultural Science, RDA)
• Myung-Ha Song(Applied Entomology Division, National Institute of Agricultural Science, RDA)
• Kwan-ho Park(Applied Entomology Division, National Institute of Agricultural Science, RDA)
• Eun-Sun Kim(Applied Entomology Division, National Institute of Agricultural Science, RDA)
• Won-Tae Kim(Applied Entomology Division, National Institute of Agricultural Science, RDA)
• Ji-young Choi(Applied Entomology Division, National Institute of Agricultural Science, RDA)