Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ɑ-pinene on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ɑ-pinene, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ɑ-pinene in a dose-dependent manner. These results suggested that ɑ-pinene may be a useful agent for inhibiting the cariogenic properties of S. mutans.

서 론
 재료 및 방법
  재료
  균주 및 배양
  S. mutans의 성장과 산 생성에 미치는 효과 측정
  S. mutans biofilm 생성에 미치는 효과 측정
  주사전자현미경(Scanning electronic microscope;SEM) 관찰
  공초점 레이저 현미경(Confocal Laser Scanning Microscopy;CLSM) 관찰
  통계처리
 결 과
  세균 성장에 미치는 효과
  산 생성에 미치는 효과
  Biofilm 형성에 미치는 효과
 고 찰
 감사의 글
 Conflict of interest
 References

• Bog-Im Park(Department. of Oral Biochemistry, School of Dentistry, Wonkwang University)
• Yong-Ouk You(Department. of Oral Biochemistry, School of Dentistry, Wonkwang University)
• Ji-Su Mo(Department. of Oral Biochemistry, School of Dentistry, Wonkwang University)
• So-Youn An(Department of Pediatric dentistry, School of Dentistry, Wonkwang University)
• Na-Young Choi(Department of Home Economics Education, Wonkwang University)
• Kang-Ju Kim(Department of Oral Microbiology & Immunology, School of Dentistry, Wonkwang University) Correspondence to