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에토프로포스 살충제를 분해하는 토양 미생물 Sphingobium sp. EP60845 분리 및 특성 KCI 등재

Isolation and characterization of ethoprophos-degrading soil bacterium Sphingobium sp. EP60845

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한국국제농업개발학회지 (The Journal of the Korean Society of International Agriculture)
한국국제농업개발학회 (The Korean Society Of International Agriculture)
초록

An organophosphorus pesticide, ethoprophos, has been widely used in agriculture to control major insect pests. As ethoprophos is a well-known neurotoxin, its accumulation in soils and groundwater is concerning worldwide. In this study, we constructed an artificial ethoprophos-enrichment soil system, and its active concentration in soils was measured by gas chromatography on 15-days intervals during 90 days after ethoprophos treatment. Furthermore, the changes in bacterial community and microorganisms responsible for efficient bioremediation were investigated while ethoprophos was degraded in soils. From 15 to 60 days after the treatment, ethoprophos was actively degraded in soils and members of genera Collimonas and Sphingobium appeared dominantly in a natural microbial community especially in 60-days-after-treatment soil. We isolated a bacterium EP60845 from this soil sample, showing significant ethoprophos biodegradation activity in vitro. When we challenged EP60845 strain into ethoprophos-enrichment soils (250 mg/kg of soil), most ethoprophos was removed within 5-days. Phylogenetic 16S rRNA gene sequence analysis and biochemical properties by API 20GN kit demonstrated that the EP60845 strain was a novel Sphingobium sp., which could be used as an efficient ethoprophos- degrading agents for bioremediation purposes.

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저자
  • 김수연(농촌진흥청 국립농업과학원) | Su-Yeon Kim
  • 백희진(농촌진흥청 국립농업과학원) | Hee-Jin Baek
  • 최수지(농촌진흥청 국립농업과학원) | Soo-Ji Choi
  • 김지선(농촌진흥청 국립농업과학원) | Ji-Seon Kim
  • 이창묵(농촌진흥청 국립농업과학원) | Chang-Muk Lee
  • 권순우(농촌진흥청 국립농업과학원) | Soon-Wo Kwon
  • 한범수(농촌진흥청 국립농업과학원) | Bum-Soo Hahn
  • 김동험(농촌진흥청 국립농업과학원) | 김동헌
  • 윤상홍(농촌진흥청 국립농업과학원) | Sang-Hong Yoon
  • 심준수(농촌진흥청 국립농업과학원) | Joon-Soo Sim 교신저자