논문 상세보기

In-Vitro, In-Vivo 동물모델에서 귀리 유래 수용성 베타-글루칸의 칼로리 제한 효과 작용기전 규명 KCI 등재

Mode of Action of Water Soluble β-Glucan from Oat (Avena sativa) on Calorie Restriction Effect In-Vitro and In-Vivo Animal Models

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/339529
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

In the current study, we investigated the inhibitory activity of water soluble β-glucan from oat (Avena sativa) against various digestive enzymes such as α-glucosidase, sucrase, maltase and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can significantly decrease the post-prandial increase of blood glucose level after a mixed carbohydrate diet. The β-glucan had the highest documented rate of small intestinal sucrase inhibitory activity (2.83 mg/mL, IC50) relevant for potentially managing post-prandial hyperglycemia. Furthermore, we evaluated the effects of β-glucan on the level of post-prandial blood glucose in animal model. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without β- glucan (100, and 500 mg/kg-body weight). The maximum blood glucose levels (Cmax) of β-glucan administration group were decreased by about 23% (from 219.06±27.82 to 190.44±13.18, p<0.05) and 10% (from 182.44±13.77 to 165.64±10.59, p<0.01) in starch and sucrose loading test, respectively, when compared to control in pharmacodynamics study. The β -Glucan administration significantly lowered the mean, maximum, and minimum level of post-prandial blood glucose at 30 min after meal. In view of the foregoing, it is felt that our findings suggest that β-glucan from oat serves to reduce post-prandial blood glucose rise secondary to slower absorption of glucose in the small intestine, via carbohydrate hydrolyzing enzymes inhibition.

목차
서 론
 재료 및 방법
  1. 시료 및 시약
  2. Rat small intestinal α-glucosidase 저해활성 분석
  3. Rat small intestinal sucrase, maltase, glucoamylase저해활성 분석
  4. Rat starch/sucrose loading test
  5. 약력학적(Pharmacodynamics) 연구
  6. 통계처리
 결과 및 고찰
  1. 인비트로 혈당상승억제 실험
  2. Rat starch/sucrose loading test
 요약 및 결론
 감사의 글
 References
저자
  • 강한나(한남대학교 식품영양학과) | Hanna Kang (Dept. of Food and Nutrition, Hannam University)
  • 권영인(한남대학교 식품영양학과) | Young-In Kwon (Dept. of Food and Nutrition, Hannam University) Corresponding author
  • 김세찬(한국폴리텍대학 바이오품질관리과) | Se-Chan Kim (Dept. Bio Quality Control, Korea Bio Polytechnic)
  • 강용수((주)유진바이오텍) | Yong Soo Kang (Eugene Biotech Co., Ltd)