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pp.1222-1228
In the current study, we investigated the inhibitory activity of water soluble β-glucan from oat (Avena sativa) against various digestive enzymes such as α-glucosidase, sucrase, maltase and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can significantly decrease the post-prandial increase of blood glucose level after a mixed carbohydrate diet. The β-glucan had the highest documented rate of small intestinal sucrase inhibitory activity (2.83 mg/mL, IC50) relevant for potentially managing post-prandial hyperglycemia. Furthermore, we evaluated the effects of β-glucan on the level of post-prandial blood glucose in animal model. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without β- glucan (100, and 500 mg/kg-body weight). The maximum blood glucose levels (Cmax) of β-glucan administration group were decreased by about 23% (from 219.06±27.82 to 190.44±13.18, p<0.05) and 10% (from 182.44±13.77 to 165.64±10.59, p<0.01) in starch and sucrose loading test, respectively, when compared to control in pharmacodynamics study. The β -Glucan administration significantly lowered the mean, maximum, and minimum level of post-prandial blood glucose at 30 min after meal. In view of the foregoing, it is felt that our findings suggest that β-glucan from oat serves to reduce post-prandial blood glucose rise secondary to slower absorption of glucose in the small intestine, via carbohydrate hydrolyzing enzymes inhibition.
재료 및 방법
1. 시료 및 시약
2. Rat small intestinal α-glucosidase 저해활성 분석
3. Rat small intestinal sucrase, maltase, glucoamylase저해활성 분석
4. Rat starch/sucrose loading test
5. 약력학적(Pharmacodynamics) 연구
6. 통계처리
결과 및 고찰
1. 인비트로 혈당상승억제 실험
2. Rat starch/sucrose loading test
요약 및 결론
감사의 글
References
