논문 상세보기
pp.1140-1148
For the purpose of developing new immunomodulatory agents from broccoli, ethanol extract (BCEE), hot water extract (BCHW), and crude polysaccharide (BCCP) were isolated from broccoli, and their immunomodulatory activities and chemical properties were examined. In the in vitro cytotoxicity analysis, BCHW and BCCP did not affect the growth of tumor cells and normal cells. Murine peritoneal macrophages stimulated with BCCP showed higher production of IL-6, IL-12, and TNF- α cytokines than those stimulated with BCHW. Also, BCHW and BCCP did not show proliferation of splenic lymphocytes. In the in vitro assay for intestinal immunomodulatory activities, only BCCP enhanced GM-CSF secretion and the bone marrow cell-proliferating activity via cells in Peyer’s patches at 1,000 μg/mL. Also, BCHW mainly contained 33.7% neutral sugars, such as arabinose, glucose, and galactose, and 30.7% uronic acid, and BCCP consisted of 42.6% neutral sugars, including arabinose, galactose, and glucose, and 50.5% uronic acid. The above results lead us to conclude that crude polysaccharide (BCCP) isolated from broccoli causes considerably high cytokine production in peritoneal macrophages and bone marrow cell proliferation, and the polysaccharide extraction process is indispensable for separation of new immunomodulatory agents from broccoli.
재료 및 방법
1. 재료
2. 브로콜리 추출물의 분리
3. 실험 동물
4. 정상세포주 및 일반세포주에 대한 세포독성
5. 대식세포(Macrophage)로부터 cytokine 생산능
6. 림프구(Lymphocyte) 증식능
7. Peyer’s patch 세포로부터 cytokine 활성능
8. 골수세포 증식능
9. 항보체 활성
10. 일반 분석 및 구성당 분석
11. 통계처리
결과 및 고찰
1. 브로콜리 유래 추출물의 정상세포주 및 종양세포주에대한 세포독성 측정
2. 브로콜리 유래 추출물의 macrophage 자극에 의한cytokine 생산능 측정
3. 브로콜리 유래 추출물의 림프구 활성능 측정
4. 브로콜리 유래 추출물의 장관면역 활성 측정
5. 브로콜리 유래 추출물의 보체계 활성화능 측정
6. 브로콜리 유래 추출물의 일반화학적 특성
요약 및 결론
감사의 글
References
