논문 상세보기
pp.27-38
This study was carried out in the Bukhan River in the summer of 2014 and 2015, to identify the relationship between geosmin and the morphological changes in Anabaena. Identification of Anabaena was conducted using morphological and molecular analyses. Anabaena in this study was similar to Anabaena circinalis, A. crass, and A. spiroides with regard to regular coils, vegetative cell, akinete shape, and size, hoever, it was distinguishabl from A. crass and A. spiroides because of its larger trichome coil size. Additionally, the sequences of phycocyanin (PC) gene from Anabaena showed a 99% genetic similarity with A. circinalis NIES-1647 strain. The coil diameter of trichome ranged from 106 to 899 μm, and the diameter and abundance showed an insignificant positive correlation (r=0.544, p<0.05). The result of relationship between the coil diameter and the cell number per 360-degree rotation was kept at 33.8±5.2 cells per 100 μm diameter despite variable diameter. The average geosmin concentrations in 2014 and 2015 were investigated to be 99 ng/L and 35 ng/L, respectively. A. circinalis cell density contributed considerably to the change in geosmin and was positively correlated with geosmin concentration (2014; r=0.599, p<0.01, 2015; r=0.559, p<0.01). Our results suggest that geosmin and coil diameter could be estimated with the help of cell density.
2. 재료 및 방법
2.1. 시료의 채집 및 분석
2.2. Anabaena의 형태 관찰 및 특징 분석
2.3. 분자생물학적 분석
3. 결과 및 고찰
3.1. Anabaena의 형태적 특성
3.2. 분자생물학적 분석
3.3. Anabaena 발생에 따른 나선의 크기변화
3.4. 팔당호 북한강 수역에서 출현한 Anabaena의발생과 지오스민과의 관계
4. 결 론
REFERENCES
