The beneficial effects of EM on plant growth, yield, quality in agriculture have been consistently demonstrated. In this study, EM of four types used is Lactobaillus sp., saccharomyces cerevisiea, Rhodobacter sp. and Bacillus sp.. Bacillus sp. inhibited Mycelium growth of the oyster mushroom in agar plate. Bacillus sp. is known to suppress plant pathogenic microorganism in soil. The other three type of EM did not inhibit mycelium growth of oyster mushroom. Also when filtering media of Lactobacillus sp. and saccharomyces cerevisiea cultivation add to liquid media(PDB) of oyster mushroom, mycelium growth was a little faster than control. In the column test, Mycelium growth of the medium inoculated with 0.5% saccharomyces cerevisiea was faster than the control. In the substrate inoculated Rhodobacter sp. was similar to the control. In the substrate inoculated Lactobacillus sp. of higher concentration, mycelium growth of the oyster mushroom was showed late than control.