The button mushroom, Agaricus bisporus is one of the most widely cultivated mushroom species. Mushroom growers inoculate compost beds with spawn, which is the vegetative mycelium and serves as the inoculum or seed for cultivating mushrooms. Various cereal grains, such as wheat, millet, barley, sorghum, brown rice, rye, and oat can be used in the spawn production of button mushroom. In this study rice seed bag spawn was compared with wheat grain bottle spawn in the spawn production. When the physical properties of two cereal grains were compared the 1000-kernel weight were 32.0g in wheat grain and 24.9g in rice seed. Number of kernels per milliliter were greater in rice seed compared with wheat grain with 24.5. Wheat grain showed 41.0% of total carbon level and 2.49% of total nitrogen level resulting 16.6 C/N ratio. Nitrogen content of rice seed was lower compared to that of wheat with 0.96 resulting 39.5 C/N. It was possible to produce 50kg of rice seed spawn in 22×49cm polypropylene filter bag with only 1~2 shakings compared to 0.45kg of wheat grain spawn in 1,000mL Ringer bottle with 4~5 shakings. Based on the results obtained, rice seed bag spawn would be appropriate substitute for wheat grain spawn in button mushroom.