This paper reports on the experimental investigation carried out to evaluate the physical optimal conditions in the absorption column to remove odorous hydrogen sulfide gas. Hydrogen sulfide gas, as a highly undesirable contaminant, is most widely emitted from environmental treatment facilities. The absorbent mixed with natural second metabolites extracted from conifer trees and chemical absorbent of 2-aminoethanol was applied to remove it via chemical neutralization. The absorbent of natural second metabolites was achieved by a removal efficiency of 20 - 40% by itself depending on the treatment conditions, but the complex absorbent mixed with 0.1% amine chemical provides the removal efficiency of 98%. The optimal removal efficiencies have been examined against the two major parameters of temperature and pH. This study shows that the aqueous solution by natural second metabolites can be used as an appropriate absorbent in the column absorbed for the removal of hydrogen sulfide gas. Young G.